- Photobleaching Techniques/ iFRAP-Inverse Fluorescence Recovery After Photobleaching (iFRAP)

It is a variant of photobleaching techniques FRAP where all the fluorescent molecules in a cell except for a small region are bleached. The loss of fluorescence from the unbleached region in the postbleach images is then analyzed. Because of the time needed to bleach large areas, this method is especially suited to analyze the dissociation parameters of molecules which are bound to an immobile structure for several seconds.