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Total Internal Reflection Fluorescence Microscopy (TIRF)

- Total Internal Reflection Fluorescence Microscopy (TIRF) Laboratorio de AFM/Fluorescencia Madrid
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Equipment
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Technical Resources

Description

Critical angle incidence of a laser beam onto the inner surface of a coverglass creates an evanescent field which illuminates the field of view with a typical depth of 100 to 200nm. Technique dedicated to probes sitting at the glass surface, and performing only at high NA, typically beyond 1.45.