Description
Critical angle incidence of a laser beam onto the inner surface of a coverglass creates an evanescent field which illuminates the field of view with a typical depth of 100 to 200nm. Technique dedicated to probes sitting at the glass surface, and performing only at high NA, typically beyond 1.45.
How this lab uses this technology
Total Internal Reflection Microscopy. 2-colors applications for membrane dynamics, cytoskeleton, in vitro studies, bio-functionalized surfaces.
Lab-specific experience
> 10 years experience,
3 staff available for experiments.
3 staff available for experiments.