Total Internal Reflection Fluorescence (TIRF, widefield variant) at Basque Resource for Advanced Light Microscopy

Total Internal Reflection Fluorescence (TIRF, widefield variant) Basque Resource for Advanced Light Microscopy Bilbao

Equipment

Technical Resources

Organizer Laboratory

BRALM

Facility Lab

Consejo Superior de Investigaciones Cientificas (CSIC)

Universidad del País Vasco / Euskal Herriko Unibertsitatea

Fundación Biofísica Bizkaia / Biofisika Bizkaia Fundazioa (FBB)

Instituto Biofisika (CSIC-UPV/EHU)

Bilbao

Instituto Biofisika (CSIC, EHU). Barrio Sarriena s/n - 48940 - Leioa - Bizkaia (Spain)

Lab Contact

Lab Members

Total Internal Reflection Fluorescence (TIRF, widefield variant)

View global technology

Utilizes an evanescent field (100–200 nm depth) to selectively excite fluorophores near the coverglass, drastically reducing background noise.