Laser Scanning Confocal Microscopy (LSCM)

Laser Scanning Confocal Microscopy

Employs a pinhole aperture and focused laser beams to collect serial optical sections, allowing for high-contrast 3D reconstruction.

Laser Scanning Confocal Microscopy

Leica Stellaris Platform

Laser Scanning Confocal Microscopy (LSCM) is an advanced fluorescence imaging technique that provides high-resolution optical sections of biological s...

Spectral imaging and spectral unmixing

Spectral imaging is an advanced fluorescence microscopy technique that records the spectral characteristics of the fluorescence emitted by a biologica...

Photoactivation, Photoconversion and Photoswitching

Photoactivation, photoconversion and photoswitching are fluorescence microscopy techniques used to selectively alter the fluorescent state of light-re...

FLAP – Fluorescence Localization After Photobleaching

Fluorescence Localization After Photobleaching (FLAP) is a ratiometric fluorescence microscopy technique used for the localized photolabelling and sub...

N&B - Number and Brightness Analysis

Number and Brightness analysis (N&B) is a fluorescence fluctuation technique used to investigate the concentration, molecular brightness, aggregat...

Quantitative colocalization analysis

Quantitative colocalization analysis is an image-analysis approach used to determine the degree of spatial association between two or more fluorescent...

Fluorescent biosensor imaging

Fluorescent biosensor imaging is an advanced microscopy approach used to visualize and quantify biochemical activities, molecular signals and physiolo...

LIGHTNING Confocal Super-Resolution Imaging

LIGHTNING is a confocal super-resolution method developed by Leica Microsystems to improve the spatial resolution, contrast and structural detail of f...

Multicolour and Three-Dimensional Imaging

Multicolour fluorescence imaging enables several molecular targets, cellular compartments or biological structures to be visualized within the same sa...

Time-Lapse and Four-Dimensional (4D) Imaging

Time-lapse fluorescence imaging is used to monitor biological processes by acquiring a sequence of images from the same specimen at defined time inter...